The effect of intermittent fasting 5:2 on IL-6 levels in obese male employees in Jakarta

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Diva Octavia Sarah
Karima Yudhistina
Erfi Prafiantini
Lily Indriani Octovia
Novi Silvia Hardiany


Background: Obesity is a condition of excessive fat accumulation in adipose tissue. This condition causes endothelial damage, increase macrophage infiltration, and inflammation in adipose tissue. Inflammation happens due to the increase of interleukin-6 (IL-6) as a proinflammatory cytokine which responsible for the occurrence of chronic diseases. Intermittent fasting is a potentially effective method for losing weight and suggested can reduce levels of proinflammatory cytokines.
Objective: The aim of this study is to determine the effect of 5:2 intermittent fasting on IL-6 cytokine levels in obese employees in Jakarta.
Methods: This study used a cross-sectional method conducted on 50 healthy male employees aged 19–52 years with a body mass index (BMI) ≥25 kg/m2. Subjects were divided into two groups, the 5:2 intermittent fasting group and the non-fasting group. Intermittent fasting 5:2 was observed every Monday and Thursday for eight weeks. IL-6 levels were obtained through examination using an enzyme-linked immunosorbent assay (ELISA) method. Statistical analysis was performed by Mann Whitney and Kruskal Wallis test.
Results: The results showed that the median of IL-6 levels decreased in fasting group from 5,10 (1.06–9.81) pg/mL to 4.64 (1.00–13.39) pg/mL and increased in non-fasting group from 3.65 (1.17–38.70) pg /mL to 6.35 (2.44–19.86) pg/mL, but the change was not significant.
Conclusion: Intermittent fasting 5:2 tend to decrease IL-6 levels in obese male employees in Jakarta although the change of IL-6 level was not significant.


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Author Biographies

Diva Octavia Sarah, Faculty of Medicine Universitas Indonesia – dr. Cipto Mangunkusumo General Hospital. Jakarta, Indonesia

Department of Nutrition

Karima Yudhistina, Faculty of Medicine Universitas Indonesia – dr. Cipto Mangunkusumo General Hospital. Jakarta, Indonesia

Department of Nutrition

Erfi Prafiantini, Faculty of Medicine Universitas Indonesia – dr. Cipto Mangunkusumo General Hospital. Jakarta, Indonesia

Department of Nutrition

Lily Indriani Octovia, Faculty of Medicine Universitas Indonesia – dr. Cipto Mangunkusumo General Hospital. Jakarta, Indonesia

Department of Nutrition

Novi Silvia Hardiany, Faculty of Medicine Universitas Indonesia – dr. Cipto Mangunkusumo General Hospital. Jakarta, Indonesia

Department of Biochemistry & Molecular Biology


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