The effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats
Abstract
Introduction: Cigarette smoke causes oxidative stress which results in reduced sperm concentration, motility and morphology, also increased levels of 8-OHdG as a marker of DNA damage. Vitamin C and E have potential role in repairing spermatozoa damages. The aim of this study was to determine the effect of vitamin C and E combination on sperm quality and cement 8-OHdG level of smoke exposed rats.Methods: This study used a post test only control group design among 18 male Wistar rats subject, aged 8 week, 150-200 grams body weight (BW). The subject was randomly divided into 3 groups, K1: control, K2: cigarettes smoke exposed, K3: cigarettes smoke exposed and given a combination of 0.045 mg/gBW vitamin C and 0.036 IU/gBW vitamin E per oral. Analysis was done on day 21 using one-way ANOVA and post-hoc LSD for sperm concentration, motility and morphology; using Kruskal-Wallis and Mann-Whitney tests for cement 8- OHdG levels.Results: The lowest sperm concentration was found in K2 (K2 32.59 million/mL, K1 47.91 million/mL, K 339.43 million/mL); the lowest normal sperm motility was found in K2 (K 238.97%, K 164.57%, K3 51.43%); the lowest normal sperm morphology was found in K2 (K2 27.56%, K 138.36%, K 331.18%); and the highest cement 8- OHdG level was found in K2 (K2 20.18ng/mL, K1 3.43ng/mL, K3 5.28ng/mL).Conclusion: Combination of vitamin C and E can improve sperm concentration, motility and morphology and decrease cement 8-OHdG levels of smoke exposed rats.Downloads
References
--
Nadeem F, Fahim A, Bugti S. Effects of cigarette smoking on male fertility. Turk J Med Sci 2012;42:1400-1405. doi: 10.3906. [Google Scholar]
Agarwal A, Virk G, Ong C, Plessis, S.S. du . Effect of Oxidative Stress on Male Reproduction. World J Mens Health 2014;32:1-17. [Google Scholar]
Agarwal A, Anandh Prabakaran S . Oxidative stress and antioxidants in male infertility: a difficult balance. International Journal of Reproductive BioMedicine 2005;3(1):1-8. [Google Scholar]
Subash P, Gurumuthy P, Sarasabharathi A, Cherian KM. 2010). Urinary 8-OHdG : A Marker of Oxidative Stress to DNA and Total Antioksidant Status in Essential Hypertension with South Indian Pupulation. Indian Journal of Clinical Biochemistry 2010;25:127-132. [Google Scholar]
Murray RK, Granner DK, Rodwell VW. . 2006;27. Indonesia. [Google Scholar]
Claudia V, Queljoe, E. de , Tendean L. Perbedaan Kualitas Spermatozoa Mencit Jantan (Mus musculus L) yang diberikan vitamin C setelah pemaparan asap rokok. Jurnal e-Biomedik (eBM 2013;1:629-634. [Google Scholar]
Intania I. Pengaruh pemberian vitamin C terhadap spermatogenesis mencit jantan strain Balb/ C yang diperi paparan asap rokok. undergraduate, Universitas Diponegoro. 2006.
Subekti SU, Juniarto ZA. Pengaruh Pemberian Vitamin E Terhadap Proses Spermatogenesis Mencit Jantan Strain Balb/C yang Diberi Paparan Asap Rokok., Universitas Diponegoro, Semarang. 2006
Wang S, Wang G, Barton BE, Murphy TF, Huang HFS. Beneficial Effects of Vitamin E in Sperm Functions in the Rat After Spinal Cord Injury. Journal of Andrology 2007;28:334-341. [Google Scholar]
Semba RD, Campbell AA, Sun K, Pee S, Akhter N, Moench-Pfanner R, et al. Paternal smoking is associated with greater food insecurity among poor families in rural Indonesia. Asia Pac J Clin Nutr 2011;20:618-616. [Google Scholar]
WHO. Global Adult Tobacco Survey : Indonesia Report. Jakarta: National Institute of Health Research and Development Ministry of Health.2012
Somwanshi SD, Madole MB, Bikkad MD, Bhavthakar SS, Ajay G, Bhgwat S. Effect of Cigarette Smoking on Sperm Count and Sperm Motility. Journal of Medical Education & Research 2012;1:30-38. [Google Scholar]
Zhang ZH, Zhu H, Li LL, Yu Y, Zhang HG, Liu RZ. Decline of cement quality and increase of leukocytes with cigarette smoking in infertile men. Iran J Reprod Med 2013;11:589-596. [Google Scholar]
Inoue T, Hayashi M, Takayanagi K, Marooka S. Oxidative DNA Damage is Induce by Chronic Cigarette Smoking, but Repaired by Abstention. Journal of Health Science 2003;49:217-220. [Google Scholar]
Fraga CG. Motchnik PA., Shigenaga MK., Helbock HJ., Jacob RA. and Ames BN. Ascorbic acid protects against endogenous oxidative DNA damage in human sperm. Proc. Natl. Acad. Sci. U.S. A 1991;88:11003-11006. [Google Scholar]
Loft S. Vistisen K., Ewertz M., Tjonneland A., Overvad K. And Poulsen HE. Oxidative DNA damage estimated by 8-hydroxydeoxy-guanine excretion in humans: Influence of smoking, gender, and body mass index. Carcinogenesis 1992;13:2241-2147. [Google Scholar]
Quratul'ainy S, Juniarto A. Pengaruh Pemberian Vitamin E Terhadap Jumlah Spermatozoa Mencit Strain Balb/C Jantan yang Diberi Paparan Asap Rokok. Universitas Diponegoro, Semarang. 2006.
Bruno RS, Traber MG. Cigarette smoke alters human vitamin E requirements. The Journal of nutrition 2005 Apr;135(4):671-4. [Google Scholar]
Kalsum U, Ilyas S, Hutahaean S. Pengaruh Pemberian Vitamin C dan E terhadap Gambaran Histologis Testis Mencit (Mus musculus L.) yang Dipajankan Monosodium glutamat (MSG). Universitas Sumatra Utara, Medan Sumatra Utara.2013.
Mostafa T. Cigarette smoking and male infertility. Journal of Advance Research 2010;1:179-186. [Google Scholar]
Piantsdosi CA. Carbon Monoxide, Reactive Oxygen Signaling, and Oxidative Stress. Free Radic Biol Med 2008;45:562-569. [Google Scholar]
Maneesh M, Jayalekshmi H. Role of Reactive Oxygen Species and Antioxidant on Pathophysiology of Male Reproduction. Indian Journal of Clinical Biochemistry 2006;21:80-89. [Google Scholar]
Batubara IVD. Wantouw B., Tendean L. Pengaruh Paparan Asap Rokok Kretek terhadap Kualitas Spermatozoa Mencit Jantan (Mus musculus. Journal e-Biomedik (eBM 2013;1:330-7. [Google Scholar]
Kefer JC, Agarwal A. E. Role of antioxidant in treatment of male infertility. International Journal of Urology 2009;16:449-457. [Google Scholar]
Hanadi BA, ElQudsi FM, Ameen HA, Sa EK. The Possible Protection Role of Antioxidants (Selenium, Vitamin E) in Reducing Smoking Effects on Testes of Albino Rats. Ass Univ Bull Environ Res 2011;14:61-73. [Google Scholar]
Moslemi MK, Tavanbakhsh S. Selenium-vitamin E suplementation in infertile men: effects on cement parameters and pregnancy rate. International Journal od General Medicine 2011;4:99-104. [Google Scholar]
--
Submitted
World Nutrition Journal is an open acces journal and under the licence of
